Poison bait is prepared to control rodents in field. To prepare this bait, 500 g leaves and inflorescence of worm killer (Aristolochia bracteata) plant are crushed. Add 100 g rice grains and 1 kg flour of pearl millet to the crushed material. Mix this material thoroughly and use it as poison bait. Put some quantity of this bait at the opening of the burrows. Rodents eat the bait and get killed.
Formulation: 500 gm leaves of (Aristolochia bracteata) plant, 100 gm rice grains and 1 kg flour of pearl millet
Ingredients: Leaves and seeds of worm killer (Aristolochia bracteata) plant, rice (Oryza sativa) grains, flour of pearl millet (Pennisetum typhoideum)
"Diarrhea, dyspnea, tympany, arching of the back, loss of condition, and loss of hair from the back were the prominent signs when Aristolochia bracteata was given orally to goats. The main lesions were haemorrhages in the lungs, heart, and kidneys, fatty change and congestion in the liver, mucoid abomasitis and enteritis and straw colored fluid in serous cavities. An increase in aspartate aminotransferase activity, ammonia and urea concentrations and a decrease in the concentrations of total protein and magnesium were detected in the serum." [Barakat, Seif eldawla & Wasfi, Ibrahim & Adam, S.. (1983). The Toxicity of Aristolochia bracteata in Goats. Veterinary pathology. 20. 611-6. 10.1177/030098588302000513.]
"The acute toxic effects of aristolochic acid (AA) were tested in rats and mice of both sexes. Oral or intravenous administration in high doses was followed by death from acute renal failure within 15 days. Histologically, the predominant features were severe necrosis affecting the renal tubules, atrophy of the lymphatic organs and large areas of superficial ulceration in the forestomach, followed by hyperplasia and hyperkeratosis of the squamous epithelium. The LD50 ranged from 56 to 203 mg/kg orally or 38 to 83 mg/kg intravenously, depending on species and sex." [Mengs, U. Acute toxicity of aristolochic acid in rodents. Arch Toxicol 59, 328–331 (1987).
"The present study is aimed at determining the hepatoprotective effect of aqueous extract of Aristolochia bracteolata leaves in paracetamol induced liver injury model in rats by administrating paracetamol on the 4th day @2g/kg b.wt. orally. Group III and IV rats were pretreated with A. bracteolata leaf extract @200mg/kg b.wt and Silymarin @200mg/kg b.wt, orally once daily for the rst 3 days respectively. After the experimental period liver tissues were collected and subjected to histopathological analysis. The histopathological studies showed marked restoration of cellular abnormalities." [Moharana, Baisakhi & Pavithra, S. & Palaninathan, Preetha & Balachandran, C. & Thangapandiyan, M. & Acharya, P. & Choudhury, Subhagata. (2017). Effect of aqueous extract of aristolochia bracteolata leaves on paracetamol induced liver toxicity in rats. Indian Veterinary Journal. 94. 70-71.]