Detail:

B S Dinesh from the Shimoga district of Karnataka soaks about 1 kg of the leaves of Mukkudaka (Lasiosophon eriocephalus) in 10 L of boiling water and keeps it overnight. Then he filters the solution and dilutes by adding about 100 L of water. The mixture is ready for spraying. While spraying, some people may develop allergic reactions including swelling of the face of water.

Knowledge Provider / Innovator: Dinesh
Agro-Ecological Zone: Nothern transition zone (KA-8)
Address: Shimoga
District: Shimoga
State: Karnataka
PIN Code 577201

Formulation: 1 kg of the leaves of Mukkudaka (Lasiosophon eriocephalus) in 10 L of boiling water
Ingredients: The shrub of Mukku Pudaka(Lasiosophon eriocephalus) and water.

---

PAS 1:

"Alcoholic extracts of Lasiosiphon have been reported to contain active secondary metabolites like Coumarins [3, 4, 5, 6]. Hence, HPTLC analysis of methanolic extract, in coumarin specific mobile phase was carried out, which suggested the presence of Coumarin (Figure 2). Coumarins are known to be antimicrobial [8]. Moreover, analysis has revealed that as compared to leaf, bark has less Coumarin content. Therefore, it can be concluded that Coumarin must have been the secondary metabolite responsible for the antimicrobial activity." [Gupta, Arvind&Oza, Goldie & Pandey, Sujeet&Durve, Annika & Bhardwaj, Sunil &Patil, B. &Kawale, Shrikant& Mishra, Neeraj&Jagdale, P.. (2010). Antimicrobial Activity of Lasiosiphoneriocephalus. Adv. In Biol. Res. ]
https://www.researchgate.net/publication/230724985_Antimicrobial_Activity_of_Lasiosiphon_eriocephalus