Problem Statement:

Rapid endotoxin entrapment and detection on surface-engineered glass substrates

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Detail:

LPS is captured from an analyte on a long-chain silane-functionalised glass substrate and tagged with gold nanoparticle (GNP) probes, surface-engineered with a narrow-spectrum antibiotic drug -- polymyxin B -- that has high specificity for LPS molecules. The signal sensitivity after GNP binding is further amplified using a 30-second silver reduction step that produces colorimetric spots in the upper femtomolar range but still visible to the naked eye. The color intensity is also quantified using an LED smartphone-based optical acquisition system and a MATLAB image-processing code. The enhancement of net sensitivity of detection beyond the clinical limit to allow pre-symptomatic (subclinical) stage monitoring and screening is performed.

Knowledge Provider / Innovator: Prasanta Kalita
Address: IIT Campus, Hauz Khas
City: Delhi
State: Delhi
PIN Code 110016

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Email: nitish.goel150@gmail.com
Contact No: 7503758094.0

Link: approach is found to be simple, sensitive, robust and cost-effective, suggesting great utility for clinical usage.
Photo Links:

• https://gyti.techpedia.in/project-detail/rapid-endotoxin-entrapment-and-detection-on-surface-engineered-glass-substrates/5498

Problem Scale: India
News Link 5:

• 
  https://www.researchgate.net/publication/318698492_Heterogeneous_endotoxin_detection_bioassay_using_drug-nanoparticle_bioconjugates_An_optimization_study