Problem Statement:

Aggregation and disaggregation of proteins and peptides http://gyti.techpedia.in/files/GYTI-BOOK-color.pdf

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Detail:

Reversion of protein or peptide aggregation is important in various domain of research at the interface of chemistry, nanoscience, and medicine. A novel class of dipeptides, termed as β-breaker dipeptides (BBDP), has been identified. The BBDP can be incorporated into the self-recognizing sequences to generate a novel class of conformational switch, which forms β-sheet at an initial stage and then converts in a controlled manner to random coil at specific condition. Such conformational switches may be used to study aggregation/disaggregation process and may find many biomedical applications relevant to aggregation related disorders. Incorporation of BBDPs in a well designed amyloidogenic peptides generates a special class of β-sheet breaker peptides those undergo a chemical change at physiological condition generating a breaker element in situ. These β-breaker peptides are shown to first incorporate into the amyloid and then disrupt it. This may be important for drug design against various amyloidoses. The reversion of peptide aggregation using chemical tricks may find application in material chemistry as well

Knowledge Provider / Innovator: Nadimpally Krishna Chaitanya
Address: Council Of Scientific And Industrial Research–National Chemical Laboratory
City: Pune
State: Maharashtra
PIN Code 411008

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Email: n.chaitanya@iitg.ernet.in
Contact No:

Link:  The β-breaker peptides exhibited characteristic signals corresponding to β-sheets when incubated in sodium acetate buffer pH 4.0 for 4 days in their CD profiles.  Also a characteristic green-gold birefringence was noticed when peptide 1 was incubated in sodium acetate buffer for 4 days, stained with Congo red and observed under polarized light (Figure 2f, left panel), indicating the presence of amyloid.  many compounds designed based on this concept may be used to study formation and disintegration of aggregation at least in vitro and may find biomedical applications for diverse amyloidoses. http://gyti.techpedia.in/files/GYTI-BOOK-color.pdf
Manufacturing Capacity: a) Turnkey solution
Problem Scale: 1) India 2) Around the world
Blog Link 5:

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  https://dx.doi.org/10.1021%2Fcn500064z
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  http://gyti.techpedia.iward-winner/2014
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  https://www.iitg.ac.in/ccd/?p=achievments

News Link 5:

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  https://pubs.rsc.org/en/content/articlelanding/2015/cc/c4cc09063b/unauth#!divAbstract
• • Levine, H. (1993) Thioflavine T interaction with synthetic Alzheimer’s disease b-amyloid
  peptides:
• Detection of amyloid aggregation in solution. Protein Sci. 2, 404−410 • Clarke, S. (1987) Propensity for spontaneous succinimide formation from aspartyl and asparaginyl residues in cellular proteins. Int. J. Pept. Protein Res. 30, 808−821.